Coding

Part:BBa_K3456001:Design

Designed by: Lina Schmidt   Group: iGEM20_Aachen   (2020-09-25)


I1-60 - ATPase Inhibitor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original IF1 amino acid sequence is: MAATALAART RQAVWSVWAM QGRGFGSESG DNVRSSAGAV RDAGGAFGKR EQAEEERYFR ARAKEQLAAL KKHHENEISH HAKEIERLQK EIERHKQSIK KLKQSEDDD

It originates from Bos taurus (Bovine). UniProtKB - P01096 (ATIF1_BOVIN)

The idea of the part comes from a paper "The affinity purification and characterization of ATP synthase complexes from mitochondria" (Runswick Michael J., Bason John V., Montgomery Martin G., Robinson Graham C., Fearnley Ian M. and Walker John E. 2013 The affinity purification and characterization of ATP synthase complexes from mitochondriaOpen Biol.3120160 http://doi.org/10.1098/rsob.120160). We designed the part as mentioned in this paper.

Source

The original IF1 amino acid sequence is: MAATALAART RQAVWSVWAM QGRGFGSESG DNVRSSAGAV RDAGGAFGKR EQAEEERYFR ARAKEQLAAL KKHHENEISH HAKEIERLQK EIERHKQSIK KLKQSEDDD. It originates from bovine.


References